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R-1 (BRL R-1): Specially constructed low voltage electromyographic amplifiers
connected to a computer are placed on a non-conducting table. The subjects have
surface (differential) electrodes attached over the upper and lower orbicular
oculi muscles of both eyes. A ground electrode is placed on the mid-forehead or
back of hand. They are seated comfortably in a dimly lit room and rest with
their eyes gently closed (relaxed). Electromyographic (EMG) recordings are made
from the lateral two thirds of the obicularis oculi muscles, bilaterally after
tapping the supraorbital notches on the right and left. The differential EMG
amplifiers permit low interference recording from electrodes without
shielding.
The specially designed low voltage EMG amplifiers permitted electromyographs
to be made in ordinary rooms even with fluorescent lights when proximity to
major sources of electrical or magnetic fields such as power line transformers
is avoided (1). For
electrical stimulation, amplifiers are modified so the electrical stimulus died
out within 8 ms so it did not interfere with the EMG signal. Electrical
stimulation is generated by variable current amplitude and duration apparatus
generally at 2.0 ms and 2.0 microamperes (MA). After preliminary testing showed
that the order of electrical or mechanical stimulation did not bias outcome,
mechanical stimulation which caused less pain and habituation preceded
electrical testing in the 19 comparisons. Blink reflexes elicited by mechanical
stimulation caused minimal discomfort to the subjects. A measurement series
consists of 10 taps to the supraorbital notch on each side to elicit BRL R-1
right and left.
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